TABLE 2.
Identities and presence of virulence genes in Shiga toxin-producing Escherichia coli (STECs) isolated from different ecosystems over 2 years from the Nijkerk cattle farm.
| Ecosystem, | Number of | Gene identification on the basis of PCR-nucleic acid lateral flow | ||||||
| sector and year* | STECs (identity) |
immunoassay (NALFIA) and confirmed by whole genome sequencing |
||||||
| O-type | stx1a | stx1b | stx2a | stx2b | ehxA | eae | ||
| RS-B-2014 | 3 (N116–118) | O150:H2 | + | + | + | + | + | + |
| GS-A-2014 | 4 (N102–105) | O76:H7 | - | - | - | - | - | + |
| GS-B-2014 | 6 (N106–111) | O150:H2 | + | + | + | + | + | + |
| GS-D-2014 | 4 (N112–115) | O150:H2 | + | + | + | + | + | + |
| SW-2014 | 2 (N119, 120) | O83:H42 | - | - | - | - | - | - |
| SM-2014 | 60, of which 10 randomly selected: | |||||||
| 5 (N7, 14, 15, 17, 20) | O150:H2 | + | + | + | + | + | + | |
| 2 (N10, 62) | O98:H21 | + | + | - | - | + | + | |
| 1 (N64) | O84:H2 | + | + | - | - | + | + | |
| 1 (N12) | O157:H7 | - | - | + | + | + | + | |
| 1(N19) | O177:H25 | - | - | + | + | + | + | |
| RS-A-2015 | 1 (N164) | O142:H25 | - | - | - | - | - | - |
| RS-B-2015 | 1 (N165) | O89:H38 | - | - | - | - | - | - |
| 1 (N166) | O128:H2 | - | - | - | - | - | + | |
| RS-C-2015 | 1 (N163) | O142:H25 | - | - | - | - | - | - |
| GS-A-2015 | 1 (N167) | O128:H2 | - | - | - | - | - | + |
| GS-B-2015 | 1 (N168) | O172:H28 | - | - | - | - | - | - |
* RS, rhizosphere soil; GS, grass shoots; SW, stream water; SM, stable manure; A through D, different field sectors; 2014, 2015, years of sampling.