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. Author manuscript; available in PMC: 2020 Jul 7.
Published in final edited form as: Thromb Haemost. 2019 May 5;119(7):1048–1057. doi: 10.1055/s-0039-1687875

Figure 1: 2D 1H-15N HSQC NMR spectrum demonstrating that FXIIIA can crosslink 15N-labeled GEE to the reactive glutamines Q237, Q328, and Q366 of Fbg αC (233–425) E396A.

Figure 1:

The glutamine side chains of Fbg αC (233–425) E396A can each be crosslinked with a 15N labeled version of the lysine mimic glycine ethyl ester. The individual crosspeaks then correspond to a proton (1H) that is covalently attached to a specific 15N label. A series of Q to inactive N mutants was used previously to match each crosspeak to a particular αC glutamine.