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. Author manuscript; available in PMC: 2020 Jul 7.
Published in final edited form as: Thromb Haemost. 2019 May 5;119(7):1048–1057. doi: 10.1055/s-0039-1687875

Figure 3: Comparing FXIII A2B2-catalyzed crosslinking of (Q237, Q328, and Q366) in WT Fbg αC (233–425) and the mutant αC E396A during two different assay time frame.

Figure 3:

Two different assay times were used for the same FXIIIa concentration. A) Reactivity of Q237 in WT Fbg αC (233–425) (black filled circle) versus the E396A mutant (black open circle) was monitored for 10 minutes in the presence of FXIII A2B2 (500 nM FXIIIA). For 2–5 minutes, the p-values were (0.011, 0.014, 0.016, 0.017), respectively. B) Glutamine reactivities in WT Fbg αC (233–425) versus the E396A mutant were monitored for 60 minutes in the presence FXIII A2B2 (500 nM FXIIIA). The individual Q residues include Q328 WT (purple triangle), Q328 E396A (golden triangle), Q366 WT (red square), Q366 E396A (blue square), Q237 WT (black filled circle), Q237 E396A (black open circle). For A) and B), the peak-height ratio method was used to calculate the amount of Q reactant left following reactions with GEE. Experiments were performed in triplicate and the results reported as mean ± SD. Asterisk (*) for p-values less than 0.05.