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. Author manuscript; available in PMC: 2020 Jul 7.
Published in final edited form as: Thromb Haemost. 2019 May 5;119(7):1048–1057. doi: 10.1055/s-0039-1687875

Figure 5: Plot comparing FXIII A2-catalyzed crosslinking of three reactive Qs in WT Fbg αC (233–425) versus αC Q237N.

Figure 5:

Glutamines reactivities in WT Fbg αC (233–425) versus the Q237N mutant were monitored for 25 minutes in the presence of 500 nM FXIIIA (pre-activated FXIII A2). The individual Q residues include Q328 WT (purple triangle), Q328 within Q237N (brown triangle), Q366 WT (red square), Q366 within Q237N (blue square), and Q237 WT (black circle). The peak-height ratio method was used to calculate the amount of Q reactant left following reactions with GEE. Experiments were performed in triplicate and the results reported as mean ± SD