Table 1. Data collection, processing, refinement and validation statistics.
| Grid type | Quantifoil 200 mesh Cu, R1.2/1.3 |
| Plunge freezer | Leica EM GP |
| Microscope | CRYO ARM 200 |
| Camera | K3 |
| Slit Width (eV) | 30 |
| Nominal magnification (kx) | 100 |
| Physical pixel size (Å) | 0.52 |
| Dose rate (e− pixel−1 s−1) | 11 |
| Total dose (e− Å−2) | 40 |
| Micrographs | 4949 |
| Particles | 257202 |
| Symmetry imposed | D2 |
| Average resolution (Å) | 1.82 |
| FSC threshold | 0.143 |
| Map sharpening B factor (Å2) | −26 |
| Refinement | |
| Initial model used (PDB entry) | 5a1a |
| Model resolution (Å) | 1.8 |
| FSC threshold | 0.5 |
| Model composition | |
| Atoms | 66138 |
| Hydrogen atoms | 30520 |
| Protein residues | 4056 |
| Waters | 3618 |
| B factors (Å2) | 9.04 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.007 |
| Bond angles (°) | 0.716 |
| Correlation coefficients | |
| Mask | 0.87 |
| Box | 0.76 |
| Validation | |
| MolProbity score | 1.12 |
| Clashscore | 3.32 |
| Poor rotamers (%) | 0.36 |
| Ramachandran plot | |
| Favored (%) | 98.12 |
| Allowed (%) | 1.78 |
| Disallowed (%) | 0.10 |