Table 4. Relationship between mRNA abundance of specific genes in eggs and embryos at selected sampling points and offspring quality parameters for the two treatments (CPE and SPE) in European eel, Anguilla anguilla, including best fitting equation and significance levels for genes, where a significant effect was found for at least one of the treatments.

				CPE			SPE
Gene	Function	Relative abundance	Quality parameter	Equation	R2	p-value	Equation	R2	p-value
dicer1	microRNA regulation	2 hpf	Cleavage abnormalities	Y = 52.67–5.57x	0.002	0.876	Y = 94.24–60.74x	0.338	0.023
epcam	cell adhesion	2 hpf	Cleavage abnormalities	Y = 67.28–9.22x	0.357	0.024	Y = 24.95–0.30x	0.004	0.831
zar1	MZT	2 hpf	Cleavage abnormalities	Y = 76.42–31.82x	0.149	0.155	Y = 62.07–35.30x	0.324	0.034
zar1	MZT	8 hpf	Survival (48 hpf)	Y = -7.26+44.41x	0.326	0.042	Y = -15.3+47.07x	0.350	0.020
sox2	MZT	8 hpf	Survival (48 hpf)	Y = 15.83+3.61x	0.729	0.0002	Y = 7.89+17.17x	0.697	0.0004
foxr1	cell division	8 hpf	Survival (48 hpf)	Y = -23.32+45.92x	0.265	0.072	Y = -56.24+75.42x	0.495	0.003
cldnd	cell adhesion	8 hpf	Survival (48 hpf)	Y = 0.75+41.59x	0.104	0.282	Y = -68.68+134.63x	0.364	0.017
phb2	normal mitochondrial function	8 hpf	Survival (48 hpf)	Y = -24.82+44.64x	0.204	0.122	Y = -32.78+49.99x	0.269	0.047
neurod4	Neurogenesis	8 hpf	Survival (48 hpf)	Y = 20.60+0.96x	0.419	0.017	Y = 12.89+3.21x-0.04x2	0.516	0.013
neurog1	Neurogenesis	8 hpf	Survival (48 hpf)	Y = 11.43+1.56x	0.672	0.0006	Y = 13.35+4.16x-0.05x2	0.509	0.014
Ccna1	cell cycle control	32 hpf	Hatch success	Y = 5.70+379.13x-478.20x2	0.524	0.024	Y = 21.41+88.96x	0.408	0.025
npm2	nuclear organization	32 hpf	Hatch success	Y = 32.02–19.88x	0.040	0.509	Y = 11.19+151.72x	0.473	0.013
oct4	MZT	32 hpf	Hatch success	Y = -5.12+545.65x-513.65x2	0.498	0.032	Y = 21.11+115.98x	0.357	0.040
neurod4	Neurogenesis	32 hpf	Hatch success	Y = 69.93–0.009x	0.048	0.473	Y = 82.06–0.03x+0.01x2	0.605	0.015
neurog1	Neurogenesis	32 hpf	Hatch success	Y = 25.03+0.004x	0.00	0.931	Y = 93.25–0.09x	0.601	0.003