Table 2.

cAMP Responses of Human PTHR1 in GP‐2.3 Cells^a

	EC50 (nM)			EMax (%)
		p versus			p versus
		PTH(1‐34)	PTHrP(1‐36)		PTH(1‐34)	PTHrP(1‐36)
PTH(1‐34)	1.4 ± 0.6	1.00	0.55	100 ± 0	1.00	0.81
PTHrP(1‐36)	1.82 ± 0.45	0.55	1.00	99 ± 3	0.81	1.00
M‐PTH(1‐11)/PTHrP(12‐36)	4.46 ± 1.27	0.088	0.13	103 ± 3	0.46	0.46
M‐PTH(1‐14)/PTHrP(15‐36)	5.18 ± 1.07	0.029	0.044	105 ± 4	0.37	0.36
M‐PTH(1‐17)/PTHrP(18‐36)	6.78 ± 1.98	0.067	0.084	105 ± 5	0.36	0.34
M‐PTH(1‐18)/PTHrP(19‐36)	6.01 ± 1.59	0.055	0.073	107 ± 5	0.28	0.26
M‐PTH(1‐22)/PTHrP(23‐36)	2.58 ± 0.26	0.12	0.20	104 ± 4	0.39	0.37
M‐PTH(1‐26)/PTHrP(27‐36)	1.79 ± 0.41	0.56	0.97	106 ± 5	0.38	0.35
M‐PTH(1‐30)/PTHrP(31‐36)	1.52 ± 0.14	0.81	0.56	106 ± 4	0.21	0.21

EC50 values are peptide concentrations that stimulated 50% of the maximum response (EMax) for that peptide. Data were derived from the area‐under‐the‐curves (AUC) of time‐course luminescence responses measured at multiple doses and calculated as a % of the maximum AUC response attained in each assay with PTH(1‐34), the average of which was 104 ± 19 × 10⁵ counts per second (cps) • min, and the corresponding basal value (not subtracted) was 0.52 ± 0.13 × 10 ^ cps • min. Data are means ± SEM of four experiments; p values determined by Student's t test compare responses to PTH(1‐34) and PTHrP(1‐36).

^acAMP responses were measured in GP‐2.3 cells (HEK293 cells stably transfected to express the hPTHR1 and GloSensor cAMP reporter).