Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (HSV-1 KOS) |
UL25 | Geneart | JQ673480.1 | |
| Strain, strain background (Escherichia coli) |
BL21(DE3) | Kerafast | LoBSTr | Chemically competent cells |
| Recombinant DNA reagent | eGFP-N2 (plasmid) |
Clontech | eGFP-N2 | |
| Recombinant DNA reagent | pKH90 (plasmid) |
PMID:24916797 | UL31 1–306 | |
| Recombinant DNA reagent | pJB02 (plasmid) | PMID:24916797 | UL34 1–220 | |
| Recombinant DNA reagent | pJB104 (plasmid) | This paper | UL25∆44 | See Materials and methods, Cloning |
| Recombinant DNA reagent | pJB118 (plasmid) | This paper | NEC-CBM | See Materials and methods, Cloning |
| Recombinant DNA reagent | pJB123 (plasmid) | This paper | UL25∆73 | See Materials and methods, Cloning |
| Recombinant DNA reagent | pED03 (plasmid) |
This paper | UL25∆44 Q72A | See Materials and methods, Cloning |
| Recombinant DNA reagent | pED05 (plasmid) |
This paper | eGFP-UL25∆73 | See Materials and methods, Cloning |
| Recombinant DNA reagent | pED13 (plasmid) |
This paper | UL25∆50 | See Materials and methods, Cloning |
| Recombinant DNA reagent | pED14 (plasmid) |
This paper | eGFP-UL25∆50 | See Materials and methods, Cloning |
| Peptide, recombinant protein | UL25∆44 | This paper | Purified from E. coli BL21(DE3) LoBSTr cells | |
| Peptide, recombinant protein | UL25∆44 Q72A | This paper | Purified from E. coli BL21(DE3) LoBSTr cells | |
| Peptide, recombinant protein | UL25∆50 Q72A | This paper | Purified from E. coli BL21(DE3) LoBSTr cells | |
| Peptide, recombinant protein | UL25∆58 Q72A | This paper | Purified from E. coli BL21(DE3) LoBSTr cells |
|
| Peptide, recombinant protein | UL25∆73 | This paper | Purified from E. coli BL21(DE3) LoBSTr cells | |
| Peptide, recombinant protein | eGFP-UL25∆50 Q72A | This paper | Purified from E. coli BL21(DE3) LoBSTr cells | |
| Peptide, recombinant protein |
eGFP-UL25∆73 | This paper | Purified from E. coli BL21(DE3) LoBSTr cells |
|
| Peptide, recombinant protein | NEC220 | This paper | Purified from E. coli BL21(DE3) LoBSTr cells |
|
| Peptide, recombinant protein | NEC-CBM | This paper | Purified from E. coli BL21(DE3) LoBSTr cells | |
| Chemical compound, drug | Cascade Blue hydrazide | Thermo Fisher Scientific |
Thermo Fisher Scientific: C687 |
|
| Software, algorithm | ImageJ | ImageJ | RRID:SCR_003070 | |
| Software, algorithm | IMOD | IMOD | RRID:SCR_003297 | |
| Other | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate | Avanti Polar Lipids | Avanti Polar Lipids:850857 | POPA |
| Other | 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine | Avanti Polar Lipids | Avanti Polar Lipids:850457 | POPC |
| Other | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine | Avanti Polar Lipids | Avanti Polar Lipids:840034 | POPS |