Video 3. The sp-ut valve fails to open during spermathecal contraction.

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Time-lapse recordings on the left are of DIC and GFP. Recordings on right are of GFP alone. Oocyte entry occurs from the left at the 15 s mark. The spermatheca was labelled by the apical junctional marker DLG-1::GFP. In the wild type (top panels), the sp-ut valve (white arrow) opened immediately to allow the oocyte to be expelled into the uterus (on the right). In pezo-1 CΔ (bottom panels), however, the DLG-1::GFP labelled sp-ut valve (white arrow) never fully opened, the oocyte was crushed as it was expelled, and ooplasmic debris was pushed out into the uterus. Images are single z planes taken every 3 s. Timing is indicated in the bottom right corner. Playback rate is 15 frames/second. Scale bars are shown in each DIC panel.