| Figure 1 |
AG404 |
pezo-1(av142[mScarlet::pezo-1]) IV, CRISPR/Cas9 edit |
| AG408 |
pezo-1(av146 [gfp::pezo-1]) IV, CRISPR/Cas9 edit |
| AG483 |
pezo-1(av182 [pezo-1::mScarlet]) IV, CRISPR/Cas9 edit |
| Figure 2 |
N2 |
Bristol (wild-type) |
| AG406 |
pezo-1(av144[N-∆]) IV, CRISPR/Cas9 edit, deletion of exon 1–13 and introns |
| AG416 |
pezo-1(av149[C-∆]) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| AG530 |
pezo-1(av149[C-∆]) IV; ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III
|
| AZ212 |
ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III |
| Figure 3 |
N2 |
Bristol (wild-type) |
| AG416 |
pezo-1(av149) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| LP598 |
dlg-1(cp301[dlg-1::mNG-C1^3xFlag]) X, CRISPR/Cas9 edit |
| AG491 |
pezo-1(av149) IV; dlg-1(cp301[dlg-1::mNG-C1^3xFlag]) X
|
| Figure 4 |
N2 |
Bristol (wild-type) |
| AG416 |
pezo-1(av149) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| Figure 5 |
UN1108 |
xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
|
AG414 |
pezo-1(av144) IV; xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II
|
|
AG415 |
pezo-1(av149) IV; xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II
|
|
AG448 |
pezo-1(av142 [mScarlet::pezo-1]) IV; xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
| Figure 6 |
N2 |
Bristol (wild-type) |
| AG406 |
pezo-1(av144) IV, CRISPR/Cas9 edit, deletion of exon 1–13 and introns |
| AG416 |
pezo-1(av149) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| AG531 |
spe-9(hc52ts) I; him-8(e1489) IV |
| BA17 |
fem-1(hc17ts) IV |
| CB1489 |
him-8(e1489) IV |
| Figure 7 |
N2 |
Bristol (wild-type) |
| AG416 |
pezo-1(av149) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| Figure 8 |
N2 |
Bristol (wild-type) |
| AG487 |
pezo-1(av190 [pezo-1::degron]) IV, CRISPR/Cas9 edit |
| AG493 |
pezo-1(av190 [pezo-1::degron]) IV; ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II; unc-119(ed3) III |
| AG494 |
pezo-1(av190 [pezo-1::degron]) IV; ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II |
| AG495 |
pezo-1(av190[pezo-1::degron]) IV; fxIs1[pie-1p::TIR1::mRuby] I |
| AG564 |
fxIs1[pie-1p::TIR1::mRuby] I |
| AG565 |
ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II; unc-119(ed3) III. |
| AG566 |
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II |
| Figure 9 |
N2 |
Bristol (wild-type) |
| AG437 |
pezo-1(av165[R2405P]) IV, CRISPR/Cas9 edit. |
| AG531 |
spe-9(hc52ts) I; him-8(e1489) IV |
| Figure 1—figure supplement 1 |
AG404 |
pezo-1(av142 [mScarlet::pezo-1]) IV, CRISPR/Cas9 edit |
| AG408 |
pezo-1(av146 [gfp::pezo-1]) IV, CRISPR/Cas9 edit |
| AG483 |
pezo-1(av182 [pezo-1::mScarlet]) IV, CRISPR/Cas9 edit |
| Figure 2—figure supplement 1 |
N2 |
Bristol (wild-type) |
| AG406 |
pezo-1(av144) IV, CRISPR/Cas9 edit, deletion of exon 1–13 and introns |
| AG416 |
pezo-1(av149) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| PS8111 |
pezo-1(sy1199) IV, CRISPR/Cas9 edit, Stop-cassette |
| PS8546 |
pezo-1(sy1398) IV, CRISPR/Cas9 edit, deletion of the first exon of isoforms i and j |
| AG570 |
pezo-1(av240) IV, CRISPR/Cas9 edit, deletion of full length of pezo-1
|
| Figure 5—figure supplement 1 |
UN1108 |
xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
| AG414 |
pezo-1(av144) IV; xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
| AG415 |
pezo-1(av149) IV; xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
| Figure 6—figure supplement 1 |
AG494 |
pezo-1(av190 [pezo-1::degron]) IV; ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II |
| AG416 |
pezo-1(av149) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| BA17 |
fem-1(hc17ts) IV |
| AG571 |
pezo-1(av149) IV; fem-1(hc17ts) IV |
| Figure 8—figure supplement 1 |
AG493 |
pezo-1(av190 [pezo-1::degron]) IV; ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II; unc-119(ed3) III |
| AG494 |
pezo-1(av190 [pezo-1::degron]) IV; ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II |
| AG495 |
pezo-1(av190[pezo-1::degron]) IV; fxIs1[pie-1p::TIR1::mRuby] I |
| Figure 8—figure supplement 2 |
AG582 |
pezo-1(av241 [gfp::pezo-1::degron]) IV, CRISPR/Cas9 edit |
| AG567 |
pezo-1(av241 [gfp::pezo-1::degron]) IV; ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II |
| AG568 |
pezo-1(av241 [gfp::pezo-1::degron]) IV; fxIs1[pie-1p::TIR1::mRuby] I |
| AG569 |
pezo-1(av241 [gfp::pezo-1::degron]) IV; ieSi65 [sun-1p::TIR1::sun-1 3′UTR + Cbr-unc-119(+)] II; unc-119(ed3) III |
| Figure 8—figure supplement 3 |
AG494 |
pezo-1(av190 [pezo-1::degron]) IV; ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II |
| Video 1 |
AG408 |
pezo-1(av146 [gfp::pezo-1]) IV, CRISPR/Cas9 edit |
| Video 2 |
N2 |
Bristol (wild-type) |
| AG406 |
pezo-1(av149)] IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| Video 3 |
LP598 |
dlg-1(cp301[dlg-1::mNG-C1^3xFlag]) X, CRISPR/Cas9 edit |
| AG491 |
pezo-1(av149) IV; dlg-1(cp301[dlg-1::mNG-C1^3xFlag]) X
|
| Video 4 |
AG406 |
pezo-1(av149) IV, CRISPR/Cas9 edit, deletion of exon 27–33 and introns |
| Video 5 |
AG448 |
pezo-1(av142 [mScarlet::pezo-1]) IV; xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
| Video 6 |
UN1108 |
xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
| AG415 |
pezo-1(av149) IV; xbIs1101 [fln-1p::GCaMP3; pRF4(rol-6D(su1006))] II |
