Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | white1118 | BloomingtonDrosophilaStock Center | BDSC: 3605 Flybase: FBst0003605 RRID:BDSC_3605 | |
| Gene (Drosophila melanogaster) | sensE1 | Nolo et al., 2000 | Flybase: FBal0098024 | From Hugo Bellen |
| Genetic reagent (Drosophila melanogaster) | sfGFP-sens [VK37] | Venken et al., 2006. From Hugo Bellen | Pacman construct containing sens gene with N-terminal 3xFlag-TEV-StrepII-sfGFP-FlAsH fusion tag inserted at 22A3 (VK37) | |
| Genetic reagent (Drosophila melanogaster) | dad-GFP [VK37] | BloomingtonDrosophilaStock Center |
BDSC: 81273 Flybase: FBti0150281 RRID:BDSC_81273 |
y w; PBac{y[+mDint2] w[+mC]=Dad GFP.FLAG} inserted at 22A3 (VK37) |
| Genetic reagent (Drosophila melanogaster) | brk-GFP [VK33] | BloomingtonDrosophilaStock Center |
BDSC: 38629 Flybase: FBti0147730 RRID:BDSC_38629 |
w1118; PBac{y[+mDint2] w[+mC]=brk GFP.FPTB} inserted at 65B2 (VK33) |
| Sequence-based reagent | GFP hybridization oligo probes | Biosearch Technologies | Custom probe set | Set of oligos with 3' modification mdC(TEG-Amino). Sequence of all oligos is in Supplementary file 1 |
| Sequence-based reagent | sens hybridization oligo probes | Biosearch Technologies | Custom probe set | Set of oligos with 3' modification mdC(TEG-Amino). Sequence of all oligos is in Supplementary file 1 |
| Sequence-based reagent | salm hybridization oligo probes | IDT | Custom probe set | Set of oligos. Sequence of all oligos is in Supplementary file 1 |
| Sequence-based reagent | omb hybridization oligo probes | IDT | Custom probe set | Set of oligos. Sequence of all oligos is in Supplementary file 1 |
| Sequence-based reagent | sd hybridization oligo probes | IDT | Custom probe set | Set of oligos. Sequence of all oligos is in Supplementary file 1 |
| Sequence-based reagent | omb intron hybridization oligo probes | IDT | Custom probe set | Set of oligos. Sequence of all oligos is in Supplementary file 1 |
| Sequence-based reagent | omb 5' exon hybridization oligo probes | IDT | Custom probe set | Set of oligos. Sequence of all oligos is in Supplementary file 1 |
| Chemical compound, drug | NHS-ester ATTO 633 dye | Sigma | #01464 | |
| Chemical compound, drug | NHS-ester ATTO 565 dye | Sigma | #72464 | |
| Chemical compound, drug | amino-11-ddUTP | Lumiprobe | A5040 | |
| Chemical compound, drug | Paraformaldehyde (powder) | Polysciences | 00380–1 | |
| Chemical compound, drug | Triton X-100 | Sigma Aldrich | T9284-500ML | |
| Chemical compound, drug | VectaShield | Vector Labs | H-1000 | |
| Chemical compound, drug | 4′,6-diamidino-2-phenylindole (DAPI) | Life Technologies | D1306 | |
| Chemical compound, drug | salmon sperm single stranded DNA | Invitrogen | #15632 | |
| Chemical compound, drug | vanadyl ribonucleoside | New England Biolabs | #S14025 | |
| Software, algorithm | MATLAB pipeline to process raw smFISH images with no prior preprocessing | This paper | https://github.com/elifesciences-publications/smfish_pipeline | |
| Other | Graces’ Insect Medium | Sigma | #69771 | Growth medium for organ culture |