Fig. 2.

Selective elevation of UPR markers with end-stage AD pathology. i) Representative images of western blots probed for a BiP, b p-PERK/PERK, c p-eIF2α/eIF2α and d p-IRE1α/IRE1α. Molecular weights and diagnosis of non-AD (C) and AD (A) are indicated, alongside images of Coomassie total protein stain as loading controls. Markers were quantified according to ii) diagnosis and iii) disease severity (low, 0–2; moderate, Braak 3–4; severe, Braak 5–6). Significant post hoc inter-group differences are indicated (*). For all phosphorylated markers, scatter plots are shown as phospho-signal adjusted for total relative to appropriate controls. BiP (n = 45), p-PERK (n = 37), p-eIF2α (n = 45), p-IRE1α (n = 23) data displayed as scatter plots with means with 95% confidence intervals, *p < 0.05