Fig. 3.

Astrocytic expression increases with end-stage AD pathology, but microglial expression remains unchanged. i) Example western blots stained for a Iba1 and b GFAP expression with molecular weights and individual diagnosis (non-AD (C), AD (A)) illustrated. Coomassie total protein loading controls are also shown. Stratified quantification of markers according to ii) diagnosis, iii) severity (low, Braak 0–2; moderate, Braak 3–4; severe, Braak 5–6) and individual Braak stages for correlation analysis (Spearman’s rank correlation (r)). Significant post hoc inter-group differences are indicated (*). Data for Iba1 (n = 46), GFAP (n = 46) given as scatter plots with mean values with 95% confidence intervals, *p < 0.05 and **p < 0.01