Fig. 1.

Summary of the experimental design. Two separate cohorts of male Lister hooded rats that received saline (1 ml/kg s.c.; Veh) or PCP (10 mg/kg) on PND 7, 9, and 11 were housed in social groups (Gr) or isolation (Iso) from weaning on PND 21. The first cohort a underwent locomotor activity, NOD, and PPI (n = 15–18 per treatment-housing combination) before balanced allocation to microsensor (n = 7–9) or western blot (n = 8–10) subgroups. The second cohort b underwent NOD on three occasions at 1–2-week intervals to receive acute vehicle (0.5% methylcellulose 1% Tween 80; 1 ml/kg i.p. 30 min before the familiarization trial), SB-399885 (10 mg/kg), or MMPIP (10 mg/kg) on separate test days in a pseudorandom order (n = 13–14 per neurodevelopmental condition), before tissue collection for immunohistochemistry