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. 2020 Mar 28;36(7):685–695. doi: 10.1007/s12264-020-00486-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Requirement of Runx1 for the development of Npy2r-persistent but not Npy2r-transient neurons. A, B Representative images of ISH with the Npy2r probe plus NF200 immunostaining in sections through lumbar DRGs from P7 Runx1^F/F control and Runx1^F/F;Wnt1-Cre mutant mice (arrows, Npy2r⁺;NF200⁺ neurons; scale bar, 100 µm). C Percentages of Npy2r⁺;NF200⁺ and Npy2r⁺;NF200⁻ among all neurons in Runx1^F/F control and Runx1^F/F;Wnt1-Cre CKO mice. D Pinprick scores evoked by a sharp steel pin glued to the tip of 1.0 g von Frey filament in Runx1^F/F control (n = 10) and Runx1^F/F;Wnt1-Cre mice (n = 6) (Student’s unpaired t-test, P = 0.2766, t₍₁₄₎ = 1.132). E, F Pinprick-evoked c-Fos expression in the ipsilateral dorsal horns in Runx1^F/F control and Runx1^F/F;Wnt1-Cre CKO mice. G Statistical comparison of c-Fos⁺ neuronal numbers in the ipsilateral dorsal horns between Runx1^F/F control and Runx1^F/F;Wnt1-Cre CKO mice (n = 6/group, one-way ANOVA with Tukey’s post-hoc analysis, F_{2, 15} =151.7, ****P < 0.0001). H, I C-Fos in the contralateral dorsal horn in Runx1^F/F control and Runx1^F/F;Wnt1-Cre CKO mice. J Statistical comparison of c-Fos⁺ neuronal numbers in the contralateral dorsal horn between Runx1^F/F control and Runx1^F/F;Wnt1-Cre CKO mice (n = 6/group, one-way ANOVA with Tukey’s post-hoc analysis, F_{2, 15} =1.075, P = 0.366).