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. 2020 Apr 10;36(7):761–777. doi: 10.1007/s12264-020-00490-x

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© The Author(s) 2020

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Fig. 2 — Changes in glycolysis and mitochondrial oxidative phosphorylation after ONC. A Statistical analysis of the mean fluorescence intensity of MitoTracker staining in retinal cells and RGCs. B Representative images and quantitative analysis of diaminobenzidine staining of cytochrome oxidase in injured and sham-operated optic nerves. C mRNA levels of tricarboxylic acid cycle enzymes in crushed optic nerves after ONC. D Quantitative analysis of lactate dehydrogenase (LDH) activity in the optic nerve and whole retina after ONC. E Representative images of LDH activity (blue/purple staining) in the retina (upper) and the optic nerve (lower) after injury [note intense and disseminated staining in the RGC layer (blue arrows); qualitative analysis in Table S1]. F mRNA levels of glycolytic enzymes in optic nerves after ONC. Scale bars in B and E, 100 μm.