Fig. 4.

Enforced expression of gSOCS3 reduces the production of inflammatory cytokines mediated by GM-CSF/IFN-γ. A TNF-α and IL-1β protein levels determined by ELISA in supernatants and lysates of cell cultures. RAW 264.7 cells were transfected with GV314-SOCS3 or GV314-vector adenovirus for 48 h following treatment with recombinant GM-CSF (50 ng/mL) or/and IFN-γ (10 ng/mL) protein for 2 h. B Expression of IL-6 and iNOS assessed by RT-PCR after cell treatment with the same approach as in A. Data are expressed as the mean ± SEM; *P < 0.05.