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Two qRT-PCR systems.
| Multiple dilution | Undiluted (25 µL) |
System A (30–50 µL) |
System B (25 µL) |
||||
|---|---|---|---|---|---|---|---|
| 0 | 2 | 4 | 5 | 6 | 2 | 3 | |
|
System (µL) Mix + RNA |
20 + 5 | 20 + 10 | 20 + 20 | 20 + 25 | 20 + 30 | 15 + 10 | 10 + 15 |
| Positive rate (%) | 100 | 100 | 100 | 100 | 96 | 100 | 100 |
Mix Premixture, RNA SARS-CoV-2 nucleic acid extraction.
