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. 2020 Jul 7;10:11133. doi: 10.1038/s41598-020-67814-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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iKras*p53* PDAC cells recapitulate oncogenic Kras* effector pathway activation and expression of membrane proteins in vitro. (a) Nuclear visualization with DAPI along with staining for phosphorylated ERK (pERK) species, indicating MAPK activity in primary tumors and iKras*p53* PDAC cells grown in 3D culture for 6 days. Scale bar 50 µm. (b) Nuclear visualization along with staining for phosphorylated S6 ribosomal protein (pS6) in primary tumors and PDAC cells in 3D culture. (c–j) DAPI staining along with visualization of membrane proteins e-cadherin and claudin-18 in primary tumor and PDAC cells in 3D culture.