Figure 1.

Efficacy of siRNAs Targeting ApoE Is Impacted by Cell Line Despite mRNA Expression

(A) Screen of 35 fully modified siRNAs in mouse N2A cells. Previously validated siRNAs targeting HTT and PPIB were used as positive controls for screening assays. (B and F) mRNA expression (relative light unit [RLU]) versus cell lysate volume using the QuantiGene branched DNA (bDNA) assay for mRNA expression in N2A (B) or primary astrocytes (F). (C and G) ApoE mRNA expression in N2A cells (C) and astrocytes (G) versus RNA concentration using qRT-PCR. (E) Screen of same 35 fully modified siRNAs in mouse primary astrocytes. (D and H) Analysis of RNA-seq datasets confirming expression of ApoE mRNA in N2A (D) and primary astrocytes (H).