Figure 2.

Increased Nuclear Localization of ApoE mRNA in Neuronal Cells

(A and C) ApoE mRNA (green) in (A) mouse N2A cells and (C) primary astrocytes (HPRT in red). (B and D) Quantification of nuclear and cytoplasmic mRNA foci in (B) N2A cells and (D) primary astrocytes (% of total foci). (E) ApoE mRNA (green) in wild-type mouse brain, with NeuN (red) marking neuronal cells. (F) Zoom (arrows) of wild-type mouse brain showing qualitative increase in ApoE mRNA in non-neuronal (NeuN-negative) cells and preferential cytoplasmic localization. Scale bars, 10 μm. Statistical analysis: t-tests using GraphPad Prism. Error bars are SD. (^∗∗∗∗ p <0.0001; ^∗∗∗ p<0.002; ^∗∗ p<0.01; ^∗ p<0.05).