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. 2020 Jun 12;21:343–353. doi: 10.1016/j.omtn.2020.06.009

Figure 1.

Figure 1

Screening of Effective gRNAs-Cas9 Targeting REV Proviral DNA

(A) Proviral DNA of reticuloendotheliosis virus (REV) reporter virus with the position of gRNAs tested in this study. (B) Flow cytometry analyses of the mean fluorescence intensity of EGFP in DF-1 cells transfected with a REV-EGFP reporter together with plasmids expressing Cas9 and a variety of gRNAs against the REV genome. (C) Comparison of antiviral disruption with single or double gRNAs performed in DF-1 cells transfected with REV-EGFP. (D) Duplex gRNAs-Cas9 in an all-in-one vector exhibited stronger inhibition of EGFP expression from the REV genome. (E) PCR genotyping of gLTR1/6 using primers to amplify the DNA fragment covering the REV LTR U3/R/U5 regions. ∗p < 0.05; ∗∗p < 0.01.