Figure 3.

Inhibition of REV Replication in CEFs by CRISPR-Cas9

Chicken embryo fibroblasts (CEFs) were transfected with Cas9 together with gLTR1/6 or empty gRNA plasmids for 24 h and then infected with REV. (A) The kinetics of REV replication was tested in CEFs. (B) The REV-infected CEFs and mock control cells were stained with anti-gp90 antibody and gp90 expression was detected by an immunofluorescence assay. (C) Western blot analysis of gp90 expression from CEFs treated with CRISPR-Cas9. (D) PCR amplification of REV proviral DNA with primers flanking the LTR region. ns, no significant difference; ∗∗p < 0.01; ∗∗∗p < 0.001.