Figure 1.

Study Design

(A) Porcine PBMCs were isolated and irradiated. Apoptotic PBMCs were further incubated in vitro for 24 h, and the supernatant of the apoptotic PBMCs (APOSEC) was collected. (B) Porcine CDCs were incubated with (apoCDC) or without APOSEC, and the cells were stored in liquid nitrogen. The secretome of CDCs and apoCDCs was analyzed by proteomics. (C) Pigs underwent 90 min occlusion of the mid-LAD and were randomized to receive CDCs (107 cells) with or without APOSEC in vitro preconditioning by intracoronary infusion during the reperfusion phase. [¹⁸F]FDG-PET-MRI imaging was performed 3 and 30 days later, and follow-up examinations were done at indicated time points.