Figure 1.

Schematic of CD5-Based and CD19-Based NSCAR Constructs

(A) NSCAR structure with CD28 transmembrane domain, truncated after two amino acids on the intracellular tail. (B and C) Bicistronic NSCAR transgenes in lentiviral vectors expressing enhanced green fluorescent protein (eGFP) and the NSCARs through the inclusion of a p2a sequence. Expression of both sequences are driven by the human ubiquitin C promoter (hUBC) with an interleukin-2 signal peptide (IL-2-SP). The CD5-NSCAR (B) includes a myc epitope tag, whereas the CD19-NSCAR (C) includes the CD8α hinge.