Figure 4.

NSCAR-Modified γδ T Cell Expansion and CD5 Downregulation

(A) Representative flow cytometry plots of γδ T cell expansion. The percentage of γδ T cells on day 7 (left) is compared to the percentage of γδ T cells on day 12 in naive cells (middle) and CD5-NSCAR-modified γδ T cells (right). (B) Percentage of γδ T cells in a population of naive (black curve) and CD5-NSCAR-modified γδ T cells (gray curve) between day of transduction (7–9) and day of cytotoxicity assay (12–13); n = 3. (C) Fold expansion of naive, GFP-modified, CD5-NSCAR-modified and CD19-NSCAR-modified γδ T cells. Naive, n = 5; GFP and CD5-NSCAR, n = 3; CD19-NSCAR, n = 2. (D) Representative flow cytometry plots of CD5 expression in CD5-NSCAR-modified (left) and CD19-NSCAR-modified γδ T cells (right). Black curve, naive; gray curve, NSCAR-modified. (E) Graphical representation of CD5 expression in naive and modified γδ T cells. Naive, n = 8; GFP and CD19-NSCAR, n = 2; CD5-NSCAR, n = 5. Statistics were performed using two-tailed Student’s t test and one-way ANOVA with Dunnett’s method to compare to the naive control group. Each replicate represents an independent donor.