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FISH schemes based on Cas9 variants.
| Method | CASFISH | CasPLA | RCasFISH |
|---|---|---|---|
| Target | highly repetitive sequences | mitochondrial DNA | mRNA |
| Time required after fixation | ~15–60 min | 8–9h | 1.5h |
| Denaturation treatment | N | N | N |
| Single-base resolution | N | Y | N |
| Single molecular imaging | N | Y | Y |
| Efficiency | ≥94% | ~25% | ≥85% |
