. 2020 Jul 8;165:112430. doi: 10.1016/j.bios.2020.112430

Table 3.

Application of CRISPR-Cas systems in sequencing analysis.

	CRISPR-DS	CRISPR-Cap	DASH	FLASH
Application	enrichment of small target regions for targeted NGS	enrichment of small target regions for targeted NGS	depletion of host-derived species for metagenomic NGS	target enrichment for multiple AMR gene sequencing
Required time for library preparation library preparation	3 days	Within 1 day	Within 1 day	Within 1 day
Fold enrichment	2000–50000^a	Several hundred^a	~3–10^b	293^b
DNA input (ng)	10–500	≥100, optimum at 1000	essentially any amount of input	0.1

Calculated by dividing the percentage of on-target ratio from sequencing data over percentage of target region in genome.

Calculated by dividing sequencing coverage of the targets from enriched sample over that from unenriched sample.