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. 2020 Jul 7;22(9):399–430. doi: 10.1016/j.neo.2020.06.002

Fig. 5.

Fig. 5

MAGEE1 overexpression inhibits cell viability in vitro. (a) The expression of MAGEE1 in the ES-2 and OVISE cell lines transfected with empty vector control (NC), MAGEE1-WT, MAGEE1-Mut1 or Magee1-Mut2 was determined by RT-PCR. (b) Western blotting of MAGEE1 expression levels in ES-2 and OVISE cells transfected with empty vector control (NC), MAGEE1-WT, MAGEE1-Mut1 or Magee1-Mut2. (c) CCK8 assay showed a significant reduction in the viability of cells transfected with MAGEE1-WT, MAGEE1-Mut1 or Magee1-Mut2 compared to cells transdfected with empty vector control (NC).