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. 2020 May 28;7(13):1903451. doi: 10.1002/advs.201903451

Figure 3.

Figure 3

Binding assay of the small‐molecule candidates with the loops. a) Pull‐down assay was performed using streptavidin magnetic bead to examine the interaction between biotin‐miR‐214‐ATF4 loop and small molecules OB‐1, OB‐2, OB‐3, and OB‐4, respectively. Small molecules OB‐1, OB‐2, OB‐3, and OB‐4 captured by biotin‐miR‐214‐ATF4 loop were examined by LC‐MS, respectively. b) Pull‐down assay was performed using streptavidin magnetic bead to examine the interaction between biotin‐miR‐214‐TRAF3 loop and small molecules OC‐1, OC‐2, and OC‐3, respectively. Small molecules OC‐1, OC‐2, and OC‐3 captured by biotin‐miR‐214‐TRAF3 loop were examined by LC‐MS, respectively.