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. 2020 May 28;9(2):CNS58. doi: 10.2217/cns-2020-0004

Figure 2. . Targeted manual validation of robot screening results (trihexyphenidyl).

Figure 2. 

(A) Cytotoxic effect of Trihexyphenidyl on GBM1 measured with normalized-to-control CellTiter-Glo® luminescence. (B) Increase of 8% of the apoptosis indicator subG1-fraction against MeOH control (p ≤ 0.001), significant increase of 7% in G0/G1 of the treated cells in the cell cycle against MeOH control (p ≤ 0.001) and flow cytometry gating. (C) Apoptosis increase and flow cytometry gating against MeOH control (p ≤ 0.001). (D) Proliferation decrease (p ≤ 0.05) supported by increase in G0/G1 in cell cycle results. All after 48 h drug exposure in GBM1 cell line. Unpaired two-sided student T test.

*p < 0.05; **p < 0.01; ***p < 0.001.

MeOH: Methanol.