Table 4.

Summary of direction of effect for behavioural tests elevated plus maze (EPM) and open field test (OFT) when compared to sham/unmanipulated controls or baseline values.

Single-Sample Method and Reference	Behaviour Test	Measure	General Direction of Effect on Measure	Timeframe for Measure
Retrobulbar
Harikrishnan et al. 2018 [12]	EPM	Anxiety	↑	24 h
	OFT	Anxiety	↑	24 h
	OFT	Locomotor activity	↓	24 h
Facial Vein
Harikrishnan et al. 2018 [12]	EPM	Anxiety	=	24 h
	OFT	Anxiety	=	24 h
	OFT	Locomotor activity	=	24 h
Moore et al. 2017 [32]	EPM	Anxiety	=	Few hours post-procedural
	OFT	Anxiety	=	Few hours post-procedural
Teilmann et al. 2014 [2]	Triple Test	Anxiety	↑	24 h
Sublingual
Harikrishnan et al. 2018 [12]	EPM	Anxiety	↑	24 h
	OFT	Anxiety	=	24 h
	OFT	Locomotor activity	↓	24 h
Tail Incision
Harikrishnan et al. 2018 [12]	EPM	Anxiety	↑	24 h
	OFT	Anxiety	=	24 h
	OFT	Locomotor activity	=	24 h
Moore et al. 2017 [32]	EPM	Anxiety	=	Few hours post-procedural
	OFT	Anxiety	=	Few hours post-procedural
Tail Vein
Teilmann et al. 2014 [2]	Triple Test	Anxiety	↓	24 h
Tail Amputation
Moore et al. 2017 [32]	EPM	Anxiety	=	Few hours post-procedural
	OFT	Anxiety	=	Few hours post-procedural