Fig. 3.

Knock-down Anillin suppresses HCC growth in orthotopic xenograft mouse model.

Mouse liver orthotopic transplantation model was constructed in four- to five-week-old male BALB/c nude mice. A. The orthotopic tumor growth was significantly suppressed in the mice treated with Anillin knock-down Hep3B cells. B. Anillin knock-down Hep3b cells induced significant decrease of the xenograft tumor weight in vivo (**P < 0.01). C–D. The xenograft tumor tissue was checked under HE staining examination. AKR1B10P1 depleted Hep3B cells induced less intrahepatic metastasis lesion and lung metastasis in mice compared with the control Hep3B cells (**P < 0.01).