Figure 5.
Autophagy model of porcine intramuscular adipocytes was constructed. (A) Monodansylcadaverine (MDC) staining of porcine intramuscular adipocytes after inducing autophagy by serum-free medium for 3 h. (B) The quantification of autophagic cells/normal cells. (C,D) RT-qPCR was carried out to detect the cell autophagic genes, ATG7 (C) and Beclin1 (D). Data were representative of means ± SEM of three independent experiments. *, p < 0.05. NC, negative control; TM, treatment.
