Fig. 4.

5-Aza-CdR treatment suppresses apoptosis and oxidative stress of SRA01/04 cells by promoting WRN expression in vitro. SRA01/04 cells were transfected with WRN siRNA or Scramble. Normal SRA01/04 cells served as control. a QRT-PCR and (b) WB were performed to detect the gene and protein expression of WRN in the modified SRA01/04 cells. The normal and modified SRA01/04 cells were consecutively treated with 100 μM H₂O₂ and 5-Aza-CdR. c QRT-PCR and d WB were performed to detect the gene and protein expression of WRN in the modified SRA01/04 cells. e CCK8 was performed to explore the cell proliferation of the modified SRA01/04 cells. f The apoptosis of the modified SRA01/04 cells was determined by flow cytometry. g The content of ROS in the modified SRA01/04 cells was detected by fluorescent probe DCFH-DA. h WB was performed to detect the expression of SOD, CAT and MDA in the modified SRA01/04 cells. (^**P < 0.01 compared with the Scramble group, ^##P < 0.01 compared with the Control or Control+Scramble group, ^$$P < 0.01 compared with the Control+WRN siRNA group)