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Fluorescence microscopy of the boundary region of the CCI lesion. The markers show (A) macrophages (ED-1), (B) astrocytes (GFAP), (C) neurons (NeuN), (D) blood vessels (nanobeads), and (E) nuclei (DAPI): a white dotted line has been added to (D) to indicate the location of the tissue boarder. These images show that the boarder of the CCI lesion is an ischemic zone with activated glia and a loss of neurons.
