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. 2020 Jul 8;20:200. doi: 10.1186/s12866-020-01877-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Immunogenic SEPs of R. akari. (a) R. akari proteins (140 μg) from whole-cell extract separated by 2-DE. 1- OmpB (A8GPL7), 2 - chaperon protein DnaK (A8GMF9), 3–60 kDa chaperonin GroEL (A8GPB6), 4–44 kDa uncharacterized protein (A8GP63), 5 – peptidoglycan associated lipoprotein (A8GPW0), 6 – superoxide dismutase (A8GNP0) (b) 2-D Western blot analysis of R. akari proteins using serum from infected rabbit (1:1000) (c) or infected patient’s serum (1:1000) (d) SDS-PAGE of selected SEPs prepared with recombinant technology. L: protein marker; lane 1–44 kDa uncharacterized protein (A8GP63) (3 μg); 2–60 kDa chaperonin GroEL (8 μg); 3- chaperon protein DnaK (6 μg). (e) Western blot analyses of recombinant (2 μg per lane) 44 kDa uncharacterized protein (lanes 1–7), 60 kDa chaperonin GroEL (lanes 8–14), and chaperon protein DnaK (lanes 15–21) probed against - 1-2, 8–9, 15–16: sera of healthy donors (negative controls, 1:1000); 3–5, 10–12, 17–19: sera of Rickettsialpox patients (1:1000), and 6–7, 13–14, 20–21: sera of patients with SFG rickettsial infection (1:1000)