Table 1.
Surface exposed proteins of R. akari
| Uniprot Accession Number | Description | mW (KDa) | pI | Identified by: | Subcellular localization | Predicted Motifs | Immunogenic | ||
|---|---|---|---|---|---|---|---|---|---|
| PSORTb | SOSUIgramN | β-barrel scorea | Signal P Type (Likelihood) | ||||||
| A8GQ33 | Putative adhesin A1C_06425 | 24.1 | 9.7 | B/T/S | outer membrane | outer membrane | 2.819a | Sec/SPI (0.9982) | – |
| A8GMA6 | 50S ribosomal protein L7/L12 | 13.0 | 5.0 | B/T/S | multiple | cytoplasmic | 2.851a | no | – |
| A8GPL7 | Outer membrane protein B | 167.9 | 5.0 | B/T/S | outer membrane | extracellular | 2.861a | Sec/SPI (0.3494) Tat/SPI (0.5171) | yes |
| A8GPB6 | 60 kDa chaperonin | 58.7 | 5.3 | B/T/S | cytoplasmic | cytoplasmic | 2.886a | no | yes |
| A8GM15 | Putative surface antigen | 48.1 | 9.6 | B | inner membrane | extracellular | 2.898a | Sec/SPI (0.9979) | – |
| A8GN82 | Malate dehydrogenase | 33.6 | 5.4 | T /S | cytoplasmic | cytoplasmic | 2.901a | no | – |
| A8GN16 | Membrane protease subunits | 33.9 | 5.5 | T /S | unknown | inner membrane | 2.902a | no | – |
| A8GPV8 | Rod shape-determining protein MreB | 37.4 | 5.3 | T /S | cytoplasmic | cytoplasmic | 2.912a | no | – |
| A8GMZ9 | ATP-dependent protease subunit HslV | 19.8 | 6.7 | T | cytoplasmic | cytoplasmic | 2.932a | no | – |
| A8GM33 | Elongation factor Ts | 33.7 | 5.2 | T /S | cytoplasmic | cytoplasmic | 2.932a | no | – |
| A8GM25 | Preprotein translocase subunit SecG | 6.5 | 9.5 | B | unknown | unknown | 2.936a | no | – |
| A8GMB0 | Probable cytosol aminopeptidase | 53.3 | 6.0 | T /S | cytoplasmic | cytoplasmic | 2.939a | no | – |
| A8GPZ4 | ATP synthase subunit beta | 51.0 | 4.6 | T /S | multiple | cytoplasmic | 2.940a | no | – |
| A8GP69 | Phospho-N-acetylmuramoyl-pentapeptide-transferase | 39.7 | 8.4 | B | inner membrane | inner membrane | 2.946a | no | – |
| A8GPZ6 | ATP synthase subunit alpha | 56.2 | 6.4 | B/T/S | cytoplasmic | cytoplasmic | 2.947a | no | – |
| A8GNC4 | Uncharacterized protein | 9.1 | 8.6 | B/S | unknown | outer membrane | 2.948a | Sec/SPII (0.9985) | – |
| A8GMH0 | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase | 30.0 | 7.0 | T /S | cytoplasmic | cytoplasmic | 2.950a | no | – |
| A8GMS9 | Heat shock protein | 18.6 | 9.2 | T /S | unknown | outer membrane | 2.954a | no | – |
| A8GP63 | Uncharacterized protein | 44.6 | 8.5 | B/T/S | unknown | extracellular | 2.957a | Sec/SPI (0.9890) | yes |
| A8GMF9 | Chaperone protein DnaK | 67.7 | 4.8 | B/T/S | cytoplasmic | cytoplasmic | 2.960aa | no | yes |
| A8GNU1 | Aminotran_5 domain-containing protein | 40.7 | 6.7 | T | cytoplasmic | cytoplasmic | 2.961a | no | – |
| A8GLV8 | ATP synthase subunit a | 27.3 | 9.0 | B | inner membrane | inner membrane | 2.962a | no | – |
| A8GNF1 | 4-hydroxy-tetrahydrodipicolinate synthase | 32.4 | 7.2 | T | cytoplasmic | cytoplasmic | 2.962a | no | – |
| A8GPZ9 | Strees induced DNA-binding protein | 16.1 | 4.9 | B/T/S | cytoplasmic | cytoplasmic | 2.963a | no | – |
| A8GLX8 | 50S ribosomal protein L9 | 19.4 | 7.7 | B/S | cytoplasmic | cytoplasmic | 2.964a | no | – |
| A8GML3 | Uncharacterized protein | 61.3 | 5.5 | T /S | outer membrane | inner membrane | 2.986 | no | – |
| A8GP43 | Protein export protein prsA | 31.4 | 9.4 | T /S | outer membrane | cytoplasmic | 2.989 | Sec/SPI (0.9958) | – |
| A8GP34 | Uncharacterized protein | 19.5 | 5.7 | B/S | unknown | extracellular | 2.990 | Sec/SPI (0.9796) | – |
| A8GMM4 | Tail-specific protease | 50.1 | 7.3 | T /S | inner membrane | outer membrane | 2.990 | Sec/SPI (0.8945) | – |
| A8GNE2 | Uncharacterized protein | 18.8 | 9.0 | T | unknown | periplasmic | 2.993 | Sec/SPI (0.9866) | – |
| A8GPM2 | Uncharacterized protein | 17.0 | 6.2 | B/S | unknown | extracellular | 3.006 | Sec/SPI (0.9979) | – |
| A8GP67 | Actin polymerization protein RickA | 59.6 | 10.1 | T /S | outer membrane | extracellular | 3.035 | no | – |
| A8GPW0 | Peptidoglycan-associated lipoprotein | 17.5 | 8.9 | T /S | outer membrane | unknown | 3.106 | Sec/SPII (0.9996) | yes |
SEPs detected using, B biotinylation, T Triton X-114, and/or S Shotgun proteomic approaches. avalues below the threshold 2.965 indicate the presence of beta-barrel structure