Table 1.
DHEAS levels in cell pellets, assayed by LC-MS/MS. SULT2B-KO and non-KO cells were incubated with 2.5 µM DHEA, 37°C for 2 hours. Harvested cell pellets were analyzed as described in (23).
| SULT2B-KO | SULT2B-positive | |||||||
|---|---|---|---|---|---|---|---|---|
| Clone #3 | Clone #6 | Clone #21 | Clone #23 | |||||
| Vehicle | +DHEA | Vehicle | +DHEA | Vehicle | +DHEA | Vehicle | +DHEA | |
| DHEA | 0.08 | 273.41 | 1.26 | 46.85 | 0.13 | 118.84 | 0.22 | 92.92 |
| (pg/mg) | 0.08 | 390.31 | 0.11 | 49.32 | 0.65 | 99.49 | 0.14 | 155.39 |
| DHEAS | 0.09 | 0.1 | 0.11 | 0.08 | 0.12 | 2.49 | 0.13 | 2.57 |
| (pg/mg) | 0.08 | 0.1 | 0.11 | 0.14 | 0.13 | 2.12 | 0.14 | 4.06 |