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. 2020 Jul 7;10:11188. doi: 10.1038/s41598-020-67702-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cumulative effects of repetitive EA stimulations on the mRNA expressions of PAR-2 (a), CGRP (b) and c-Fos (c) in the spinal cord of the goats (Mean ± SD, n = 6). Quantitative real-time PCR (qRT-PCR) was performed to measure the mRNA expressions of PAR-2, CGRP and c-Fos (relative to GAPDH) in T₁₁ thoracic spinal cord dorsal horns. (a) PAR-2 mRNA; P = 0.000, Sham-A or VH group versus Control group; P = 0.000, VH group versus Sham-A group; P = 0.000, EA group versus VH group [F (3, 20) = 675.15, P = 0.000]. (b) CGRP mRNA; P = 0.000, Sham-A or VH group versus Control group; P = 0.000, VH group versus Sham-A group; P = 0.000, EA group versus VH group [F (3, 20) = 1,437.07, P = 0.000]. (c) c-Fos mRNA; P = 0.000, Sham-A or VH group versus Control group; P = 0.000, VH group or EA group versus Sham-A group; P = 0.000, EA group versus VH group [F (3, 20) = 930.32, P = 0.000]. **P < 0.01, VH/Sham-A/EA group versus Control group. ⁺⁺P < 0.01, VH/EA group versus Sham-A group. ^##P < 0.01, VH group versus EA group. One-way ANOVA followed by Bonferroni’s post-hoc test.