Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 7;10:11140. doi: 10.1038/s41598-020-67935-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analysis of miRNAs by RNA isolation kit. (A) Multivariate analysis: PLS-DA of miRNAs according to the RNA isolation kit used. Red triangles and green crosses represent samples isolated with Norgene and miRNeasy (Qiagen), respectively. Class membership was represented by a 95% confidence ellipses calculated from PLS-DA scores. (B) Variable Importance in Projection (VIP) of the top 15 miRNAs, which represents the relative contribution of miRNAs to the variance between the two RNA isolation kits. The higher the VIP score, the better the contribution of the miRNA to the group separation. The green and red boxes on the right indicate whether the miRNA expression is increased (red) or decreased (green) on each group. (C) Heatmap of the 30 top miRNAs in the PLS-DA. Columns represent each sample, while rows correspond to miRNAs. The miRNA clustering tree is shown on the left, and the sample clustering tree is shown at the top. A higher presence of miRNAs is represented by red squares and lower presence by blue squares.