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. 2020 Jul 7;10:11148. doi: 10.1038/s41598-020-67594-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article is included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered spermiogenesis of Slc16a2−/− rat. (A) Representative images of H&E stained cauda epididymis sections of Slc16a2+/+ and Slc16a2−/− rats at p49. Scale bar = 50 μm. (B–E) CASA analysis of spermatozoa of Slc16a2+/+ and Slc16a2−/− rats at p49. (B) Spermatozoa concentration; (C) spermatozoa motility; (D) spermatozoa average path velocity (VAP); (E) spermatozoa straight line velocity (VSL); (F) spermatozoa curvilinear velocity (VCL). n = 12, P < 0.001;*** P < 0.0001;****.