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. 2020 Jul 7;10:11139. doi: 10.1038/s41598-020-68014-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analyses of DvSSJ1 siRNA expression in transgenic plants. Size distribution and strand orientation of DvSSJ1 small RNAs between 15 and 41 nucleotides were collected from root tissue under the control of the UBI promoter and showed in the top panel. Sense (red) and antisense (blue) of DvSSJ1 siRNAs were mapped to 210 bp DvSSJ1 fragment (Bottom panel). DvSSJ1 siRNA reads were visualized using Integrative Genomics Viewer software 2.8 (Broad Institute, Cambridge, MA, USA) (https://software.broadinstitute.org/software/igv/).