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. 2020 Jul 7;10:11139. doi: 10.1038/s41598-020-68014-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterizations of transgenic plants expressing DvSSJ1 siRNAs and dsRNA. T0 single copy plants were grown in the greenhouse and root tissues were collected for siRNA-1 (A), siRNA-2 (B) and 210-bp dsRNA (C) quantification as described in Method; (D) The DvSSJ1 transgenic lines and non-transgenic control (NTC) were selected for T0 greenhouse assay. Eight plants per DvSSJ1 dsRNA line and NTC plants were assayed for WCR feeding damage. The CRW nodal injury score (mean ± SD) of dsRNA line was significantly different (P value < 0.0004–0.004) between the NTC and siRNA transgenic lines. No significant difference between two small RNA constructs and non-transgenic control (NTC).