Table 1.
Sequence comparison of DvSSJ1 homologous.
| Order | Name | Percent identity to DvSSJ1 dsRNA | Number of SNPs | Number of 21 nt matches (or longest nt sequence)* |
|---|---|---|---|---|
| Coleoptera | Diabrotica virgifera virgifera | 100 | 0 | 190 |
| Diabrotica longicornis | 97.1 | 6 | 135 | |
| Diabrotica undecimpunctata | 92.9 | 15 | 79 | |
| Phyllotreta cruciferae | 77.6 | 47 | 0 (20) | |
| Phyllotreta striolata | 76.2 | 50 | 0 (19) | |
| Leptinotarsa decemlineata | 73.3 | 56 | 0 (12) | |
| Tribolium castaneum | 69.5 | 64 | 0 (11) | |
| Zophobas morio | 69 | 65 | 0 (10) | |
| Epilachna varivestis | 67.6 | 68 | 0 (12) | |
| Tenebrio molitor | 65.2 | 73 | 0 (10) | |
| Dalotia coriaria | 64.3 | 75 | 0 (8) | |
| Cryptolaemus montrouzieri | 63.3 | 77 | 0 (8) | |
| Coleomegilla maculata | 61.9 | 80 | 0 (13) | |
| Hymenoptera | Apis mellifera | 68.1 | 67 | 0 (10) |
| Hemiptera | Orius insidious | 61.9 | 80 | 0 (11) |
| Lepidoptera | Vanessa cardui | 64.8 | 74 | 0 (8) |
| Ostrinia nubilalis | 64.2 | 79 | 0 (9) | |
| Spodoptera fugiperda | 62.9 | 78 | 0 (11) | |
| Cydia pomonella | 60.5 | 83 | 0 (8) | |
| Helicoverpa zea | 60 | 84 | 0 (8) |
The sequences of twenty species, representing four families within the order Coleoptera, four families within the order Lepidoptera, and one family each from the order Hymenoptera and Hemiptera were compared to the 210 bp sequence from the WCR SSJ gene to determine the percent similarity, number of single nucleotide polymorphisms (SNPs), and the number of 21nt matches.
*Longest nucleotide sequence showing 100% match to DvSSJ1 210 sequence.