Table 3.
Analysis of WCR mortality for dsRNA specificity data.
| Treatment description | Mortality (%) | 95% Confidence limit | FDR adjusted P value versus 21 bp DvSSJ1 in GFP | Fisher's test P value versus GFP control |
|---|---|---|---|---|
| RNAse free H2O | 17.2 | 5.85–35.8 | 0.0006* | 0.2299 |
| GFP control (210 bp GFP) | 34.5 | 17.9–54.3 | 0.0361* | – |
| DvSSJ1 21 bp embedded within 210 bp GFP | 68.0 | 46.5–85.1 | – | 0.0281* |
| DvSSJ1 1 bp point mutation (loc 1) | 55.2 | 35.7–73.6 | 0.4073 | 0.1864 |
| DvSSJ1 2 bp point mutations (locs 1, 3) | 20.0 | 7.71–38.6 | 0.0008* | 0.2516 |
| DvSSJ1 3 bp point mutations (locs 1, 3, 5) | 23.3 | 9.93–42.3 | 0.0018* | 0.3985 |
| DvSSJ1 4 bp point mutations (locs 1, 3, 5, 7) | 17.2 | 5.85–35.8 | 0.0006* | 0.2299 |
| DvSSJ1 5 bp point mutations (locs 1, 3, 5, 7, 9) | 17.9 | 6.06–36.9 | 0.0006* | 0.2299 |
| DvSSJ1 1 bp point mutation (loc 11) | 16.7 | 5.64–34.7 | 0.0006* | 0.1432 |
| DvSSJ1 210 bp dsRNA | 92.9 | 76.5–99.1 | 0.0378* | < 0.0001* |
Increasing single nucleotide polymorphisms (SNPs) were introduced in various locations (loc) within the DvSSJ1 21-mer and fed to WCR over 14 days to assess WCR mortality.
*A statistically significant difference (P value < 0.05) was observed.