Table 4.

Summary analysis of WCR weight results for dsRNA specificity data.

Treatment description	Mean weight (mg) (95% confidence interval)	Range (mg)	Adjusted P value versus 21 bp DvSSJ1 in GFP	P value versus GFP control
RNAse free H2O	1.93 (1.61–2.26)	0.4–3.5	< 0.0001*	0.9374
GFP control (210 bp GFP)	1.95 (1.59–2.31)	0.2–3.3	< 0.0001*	–
DvSSJ1 21 bp embedded within 210 bp GFP	0.375 (-0.183–0.933)	0.1–1.1	–	< 0.0001*
DvSSJ1 1 bp point mutation (loc 1)	1.03 (0.593–1.47)	0.3–2.3	0.2387	0.0016*
DvSSJ1 2 bp point mutations (locs 1, 3)	1.23 (0.903–1.55)	0.1–2.3	0.0445*	0.0034*
DvSSJ1 3 bp point mutations (locs 1, 3, 5)	1.85 (1.52–2.18)	0.3–3.2	< 0.0001*	0.6728
DvSSJ1 4 bp point mutations (locs 1, 3, 5, 7)	1.62 (1.29–1.94)	0.2–3.0	0.0012*	0.1727
DvSSJ1 5 bp point mutations (locs 1, 3, 5, 7, 9)	2.00 (1.67–2.33)	0.4–2.8	< 0.0001*	0.8486
DvSSJ1 1 bp point mutation (loc 11)	1.37 (1.05–1.68)	0.3–3.0	0.0130*	0.0173*
DvSSJ1 210 bp dsRNA	1.40 ± 0.707	0.9–1.9	–	–

Increasing single nucleotide polymorphisms (SNPs) were introduced in various locations (loc) within the DvSSJ1 21-mer and fed to WCR over 14 days to assess WCR weight.

*A statistically significant difference (P value < 0.05) was observed.