Figure 3.

The Three SWI/SNF Complex Assemblies Have Distinct Regulatory Roles for Foxp3 Expression in Treg Cells

(A) A diagram showing three different variants of SWI/SNF complexes: BAF, ncBAF, and PBAF. BAF-specific subunits (Arid1a and Dpf1–Dpf3) are colored blue, ncBAF-specific subunits (Brd9, Smarcd1, Gltscr1l, and Gltscr1) are colored orange, and PBAF-specific subunits (Pbrm1, Arid2, Brd7, and Phf10) are colored green. Shared components among complexes are colored gray. Also shown is an immunoprecipitation assay of Arid1a, Brd9, Phf10, and Smarca4 in Treg cells. The co-precipitated proteins were probed for shared subunits (Smarca4, Smarcc1, and Smarcb1), BAF-specific Arid1a, ncBAF-specific Brd9, and PBAF-specific Pbrm1.

(B) FACS histogram of Foxp3 expression in Treg cells after sgRNA targeting of the indicated SWI/SNF subunits.

(C) MFI of Foxp3 after sgRNA targeting of the indicated SWI/SNF subunits. Data represent mean and standard deviation of biological replicates (n = 3–21).

(D) Principal-component analysis of RNA-seq data collected from Treg cells transduced with guides against the indicated SWI/SNF subunits. In cases where two independent guides were used to target a gene, the second guide for targeting the gene is indicated as “-2.”

(E) MFI of Foxp3 expression in Treg cells after treatment with DMSO or 0.16–10 μM dBRD9 for 4 days.

Data represent mean ± SD. Statistical analyses were performed using unpaired two-tailed Student’s t test (non-significant [ns], p ≥ 0.05; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001). See also Figure S5.