REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD4-Alexa fluor 700	Thermo Fisher	Cat#56-0042-82; RRID: AB_494000
Anti-CD4-PerCP-Cy5.5	TONBO	Cat#65-0042-U100; RRID: AB_2621876
Anti-CD8-PE	Thermo Fisher	Cat#12-0081-85; RRID: AB_465532
Anti-CD8-BV510	Biolegend	Cat#100752; RRID: AB_2563057
Anti-CD45.1-BV605	Biolegend	Cat#110735; RRID:AB_11124743
Anti-CD45.2-Alexa 700	Biolegend	Cat#109822; RRID:AB_493731
Anti-Foxp3-eFluor 450	Thermo Fisher	Cat#48-5773-82; RRID:AB_1518812
Anti-NGFR-PE	Biolegend	Cat#345106; RRID:AB_2152647
Anti-NGFR-APC	Biolegend	Cat#345108; RRID:AB_10645515
Anti-Thy1.1-PE	Thermo Fisher	Cat#12-0900-83; RRID:AB_465774
Anti-CD44-BV650	Biolegend	Cat#103049; RRID:AB_2562600
Anti-CD62L-BV605	Biolegend	Cat#104438; RRID:AB_2563058
Anti-IFNg-APC	Thermo Fisher	Cat#17-7311-82; RRID:AB_469504
Ghost Viability Dye Red 780	TONBO	Cat#13-0865-T100
Anti-Foxp3	In-house	n/a
Anti-BRG1/SMARCA4	Abcam	Cat#110641; RRID:AB_10861578
Anti-BAF155/SMARCC1	Santa Cruz	Cat#sc-10756; RRID:AB_2191997
Anti-BAF47/SMARCB1	Santa Cruz	Cat#sc-166165; RRID:AB_2270651
Anti-Brd9	Active Motif	Cat#61537; RRID:AB_2614970
Anti-Pbrm1	Bethyl	Cat#A301-591A; RRID:AB_1078808
Anti-Phf10	Thermo Fisher	Cat#PA5-30678; RRID:AB_2548152
Anti-Arid1a	Santa Cruz	Cat#sc-32761; RRID:AB_673396
Anti-Histone H3K27ac	Abcam	Cat#ab4729; RRID:AB_2118291
Anti-IgG	Cell Signaling	Cat#2729S; RRID:AB_1031062
Anti-mouse secondary	Thermo Fisher	Cat#A21058; RRID:AB_2535724
Anti-rabbit secondary	Thermo Fisher	Cat#SA535571; RRID:AB_2556775
Anti-mouse IL2	BIO-X-CELL	Cat#BE0043 RRID:AB_1107702
Recombinant DNA
pSIR-dsRed-Express2	Addgene	Cat#51135; RRID:Addgene_51135
pSIRG-NGFR	This paper	n/a
pSIRG-GFP	This paper	n/a
pCL-Eco	Addgene	Cat#12371; RRID:Addgene_12371
lentiCRISPRv2-Brie library	Addgene	Cat#73632
pSIRG-NGFR-Brie library	This paper	n/a
pSIRG-NGFR-sgFoxp3 Target: TCTACCCACAGGGATCAATG	This paper	n/a
pSIRG-NGFR-sgCbfb Target: GCCTTGCAGATTAAGTACAC	This paper	n/a
pSIRG-NGFR-sgDnmt1 Target: TAATGTGAACCGGTTCACAG	This paper	n/a
pSIRG-NGFR-sgArid1a Target: GCAGCTGCGAAGATATCGGG	This paper	n/a
pSIRG-NGFR-sgArid1a-2 Target: TACCCAAATATGAATCAAGG	This paper	n/a
pSIRG- NGFR-sgArid1b Target: TGAGTGCAAAACTGAGCGCG	This paper	n/a
pSIRG-NGFR-sgArid1b-2 Target: CAGAACCCCAACATATAGCG	This paper	n/a
pSIRG-NGFR-sgDpf1 Target: TCTTCTACCTCGAGATCATG	This paper	n/a
pSIRG-NGFR-sgDpf2 Target: GAAGATACGCCAAAGCGTCG	This paper	n/a
pSIRG-NGFR-sgPbrm1 Target: AAAACACTTGCATAACGATG	This paper	n/a
pSIRG-NGFR-sgPbrm1-2 Target: CAATGCCAGGCACTACAATG	This paper	n/a
pSIRG-NGFR-sgArid2 Target: ACTTGCAGTAAATTAGCTCG	This paper	n/a
pSIRG-NGFR-sgBrd7 Target: CAGGAGGCAAGCTAACACGG	This paper	n/a
pSIRG-NGFR-sgPhf10 Target: GTTGCCGACAGACCGAACGA	This paper	n/a
pSIRG-NGFR-sgBrd9 Target: ATTAACCGGTTTCTCCCGGG	This paper	n/a
pSIRG-NGFR-sgBrd9-2 Target: GGAACACTGCGACTCAGAGG	This paper	n/a
pSIRG-NGFR-sgGltscr1 Target: GTTCTGTGTAAAATCACACT	This paper	n/a
pSIRG-NGFR-sgGltscr1l Target: ATGGCTTTATGCAACACGTG	This paper	n/a
pSIRG-NGFR-sgSmarcd1 Target: CAATCCGGCTAAGTCGGACG	This paper	n/a
pSIRG-NGFR-sgEny2 Target: AGAGCTAAATTAATTGAGTG	This paper	n/a
pSIRG-NGFR-sgAtxn7l3 Target: GCAGCCGAATCGCCAACCGT	This paper	n/a
pSIRG-NGFR-sgUsp22 Target: GCCATCGACCTGATGTACGG	This paper	n/a
pSIRG-NGFR-sgCcdc101 Target: CCAGGTTTCCCGATCCAGAG	This paper	n/a
pSIRG-NGFR-sgTada3 Target: GAAGGTCTGTCCCCGCTACA	This paper	n/a
pSIRG-NGFR-sgTada1 Target: TTTCCTTCTCGACACAACTG	This paper	n/a
pSIRG-NGFR-sgTaf6l Target: TCATGAAACACACCAAACGA	This paper	n/a
pSIRG-NGFR-sgSupt20 Target: TTAGTAGTCAATCTGTACCC	This paper	n/a
pSIRG-NGFR-sgSupt5 Target: GATGACCGATGTACTCAAGG	This paper	n/a
pSIRG-NGFR-sgNT Target: AAAAAGTCCGCGATTACGTC	This paper	n/a
pSIRG-GFP-sgBrd9 Target: ATTAACCGGTTTCTCCCGGG	This paper	n/a
pSIRG-GFP-sgNT Target: AAAAAGTCCGCGATTACGTC	This paper	n/a
MSCV-IRES-GFP (MIGR)	Addgene	Cat#27490; RRID:Addgene_27490
MIGR-Foxp3	The laboratory of Alexander Rudensky	n/a
Chemicals, Peptides, and Recombinant Proteins
Human IL-2	Peprotech	Cat#200-02
Mouse IL-2	Biolegend	Cat#575408
dBRD9	Tocris	Cat#6606
NEBuilder HIFI assembly	NEB	Cat#E2621S
BbsI-HF	NEB	Cat#R3539S
Q5 High-Fidelity DNA polymerase	NEB	Cat#M0491
Ficoll-Paque 1.084	GE Health	Cat#17-5446-02
FuGENE 6 HD transfection reagent	Promega	Cat#E2311
Foxp3 Fix/Perm buffer	Thermo Fisher	Cat#00-5523-00
CellTrace Violet	Thermo Fisher	Cat#C34571
Cell Lines
HEK293T	ATCC	Cat#CRL-11268
MC38	The laboratory of Susan Keach	n/a
Mouse Strains
Rosa26-LSL-Cas9 mice	The Jackson Laboratory	Cat#024857
Foxp3Thy1.1 reporter mice	Liston et al., 2008	n/a
C57BL.6 Ly5.1+ congenic mice	The Jackson Laboratory	Cat#002014
Rag1−/− mice	The Jackson Laboratory	Cat#002216
Software and Algorithms
MAGeCK	https://sourceforge.net/p/mageck/wiki/Home/; Li et al., 2014a	n/a
MAGeCK-VISPR	https://bitbucket.org/liulab/mageck-vispr; Li et al., 2015	n/a
EnhancedVolcano R script	https://github.com/kevinblighe/EnhancedVolcano	n/a
sgRNA distribution histogram R script	Shifrut et al., 2018	n/a
Metascape	Zhou et al., 2019	n/a
HOMER	http://homer.ucsd.edu/homer/	n/a
Cluster 3.0	http://bonsai.hgc.jp/∼mdehoon/software/cluster/software.htm	n/a
Gene Set Enrichment Analysis (GSEA)	https://www.gsea-msigdb.org/gsea/index.jsp; Mootha et al., 2003; Subramanian et al., 2005	n/a
Cutadapt	http://cutadapt.readthedocs.io/en/stable/	n/a
Samtools	http://htslib.org; Li et al., 2009	n/a
Picard	http://broadinstitute.github.io/picard	n/a
BWA Aligner	http://bio-bwa.sourceforge.net; Li and Durbin, 2009	n/a
Macs2	http://pypi.org/project/MACS2	n/a