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. 2020 Jul 8;9(7):e1147. doi: 10.1002/cti2.1147

Figure 1.

Figure 1

Gene constructs and surface expression on T and NK cells of first‐generation NKp44‐based CARs. (a) A series of first‐generation NKp44‐based CARs shared the ligand‐binding domain of NKp44. (b) Surface expression levels of the first‐generation NKp44‐based CARs in transduced T cells and NK cells are presented. The horizontal axis represents levels of GFP. The vertical axis represents the surface expression levels of NKp44‐based CAR demonstrated by PE‐conjugated anti‐NKp44 monoclonal antibody in transduced T cells. Same antibody detects NKp44‐based CAR expressed by transgene as well as intrinsic NKp44 in the transduced NK cells. Expression levels are much higher for NKp44‐based CAR (shown in the right upper quadrant) than for intrinsic NKp44 (the left upper and lower quadrant). The data are representative of at least three independent experiments using different peripheral blood donors. EH, extracellular hinge domain; IC, intracellular domain; Ig‐like, immunoglobulin‐like domain; TM, transmembrane domain.